gi_545778205_gb_U000_104

cmsearch-Rfam

Family :

Leu_leader

Id:

RF00512

Type:

Cis-reg; leader;

Taxonomy:

Eukaryota; Bacteria;

Ontology:

GO:0009098 leucine biosynthetic process

Description:

At least 6 amino acid operons are known to be regulated by attenuation [1]. In each
a leader sequence of 150-200 bp is found upstream of the first gene in the
operon. This leader sequence can assume two different secondary structures known as the terminator and
the anti-terminator structure. In each case the leader also codes for very short peptide sequence
that is rich in the end product amino acid of the operon. The terminator structure
is recognised as a termination signal for RNA polymerase and the operon is not transcribed.
This structure forms when the cell has an excess of the regulatory amino acid and
ribosome movement over the leader transcript is not impeded [1]. When there is a deficiency
of the charged tRNA of the regulatory amino acid the ribosome translating the leader peptide
stalls and the antiterminator structure can form. This allows RNA polymerase to transcribe the operon
[1]. In the Rfam alignment the consensus structure is of the terminator. The alternative consensus
structure is the antiterminator structure.

Score:

26.0

E-value:

0.0064

From sequence:

2653330

To sequence:

2653385

Alignments:

                                                                                            NC
                               :::<-<<<<~~~~~~>>>>->----------<<<<<<<<<<<____>>>>>>>>>>>::: CS
               RF00512       1 CuggAgcug*[94]*cagcccacauuAaaaaaAAacCCGCGCuauUGCGCGGguUUuUUU 148    
                                   A::U:      :A::+ ACA   AA+AAAAA:CCGC   AUU  GCGG:UUUUU U
  gi_545778205_gb_U000 2653330 AGUGAAAUA*[ 2]*UAUUUUACAGGCAAUAAAAAACCGCCGAAUUUGGCGGUUUUUUAU 2653385
                               ******997...9..6999***************************************** PP

RNAfold
>gi_545778205_gb_U000_104
CGCAUGGAGUGAAUGGGGCGCGCGGGCAGAUUUACCGGUUGAGCCAGUGA
AAUAAGUAUUUUACAGGCAAUAAAAAACCGCCGAAUUUGGCGGUUUUUUA
UUGCUAGUCUGGUUCGCGGCCUUUCCAGCAGGUUGACUUGUGUUACA
.((.(((((......((((.((((((((((((....(((...))).(((((((....))))))).((((((((((((((((((....))))))))))))))))))))))).))))))))))))))))))....((((....)))).. (-57.60)

Length sequence

: 147

Length match:

54