gi_545778205_gb_U000_105

cmsearch-Rfam

Family :

Leu_leader

Id:

RF00512

Type:

Cis-reg; leader;

Taxonomy:

Eukaryota; Bacteria;

Ontology:

GO:0009098 leucine biosynthetic process

Description:

At least 6 amino acid operons are known to be regulated by attenuation [1]. In each
a leader sequence of 150-200 bp is found upstream of the first gene in the
operon. This leader sequence can assume two different secondary structures known as the terminator and
the anti-terminator structure. In each case the leader also codes for very short peptide sequence
that is rich in the end product amino acid of the operon. The terminator structure
is recognised as a termination signal for RNA polymerase and the operon is not transcribed.
This structure forms when the cell has an excess of the regulatory amino acid and
ribosome movement over the leader transcript is not impeded [1]. When there is a deficiency
of the charged tRNA of the regulatory amino acid the ribosome translating the leader peptide
stalls and the antiterminator structure can form. This allows RNA polymerase to transcribe the operon
[1]. In the Rfam alignment the consensus structure is of the terminator. The alternative consensus
structure is the antiterminator structure.

Score:

28.1

E-value:

0.0019

From sequence:

2976396

To sequence:

2976305

Alignments:

                                     v v                                     v v             vv                  NC
                               :::<-<<<<<-<<<.<<<<------~~~~~~-----.>>>>>>>->>>>>->----------<<<<<<<<<<<____>>>> CS
               RF00512       1 CuggAgcugguuuc.augccuCaua*[65]*AUUCA.gcaugaauccagcccacauuAaaaaaAAacCCGCGCuauUGCGC 138    
                                ++G G U :U::C :: :+ ++ A      A UCA : ::G::U: A C CA  ++ +A A  A:CC:GCGC ++UGCGC
  gi_545778205_gb_U000 2976396 AAAGUGGUGAUCCCaGAGGUAUUGA*[ 7]*AGUCAaCUUCGGGUUGAGCACAUGAAUUACACCAGCCUGCGCAGAUGCGC 2976315
                               **************65555555444...8..5566736668**************************************** PP

                                    vv    NC
                               >>>>>>>::: CS
               RF00512     139 GGguUUuUUU 148    
                               :GG:U  UUU
  gi_545778205_gb_U000 2976314 AGGUUUUUUU 2976305
                               ********** PP

>>

RNAfold
>gi_545778205_gb_U000_105
GUUACUGUUACAGAUUGAUGACCGGCAAAAAAAACCUGCGCAUCUGCGCA
GGCUGGUGUAAUUCAUGUGCUCAACCCGAAGUUGACUUCACCUAUCAAUA
CCUCUGGGAUCACCACUUUAGCAACCUGAAGCCAAACGCCACCAGCG
....((((((........))).))).........(((((((....)))))))((((((........(((.(((((.....)))))...))).............(((.....)))((((((....))))))........)))))).. (-34.30)

Length sequence

: 147

Length match:

90