rnpB

cmscan-Rfam

Family :

RNaseP_bact_a

Id:

RF00010

Type:

Gene; ribozyme;

Taxonomy:

Eukaryota; Bacteria;

Ontology:

GO:0008033 tRNA processingRF00010

Description:

Ribonuclease P (RNase P) is a ubiquitous endoribonuclease, found in archaea, bacteria and eukarya as well
as chloroplasts and mitochondria. Its best characterised activity is the generation of mature 5'-ends of
tRNAs by cleaving the 5'-leader elements of precursor-tRNAs. Cellular RNase Ps are ribonucleoproteins. RNA from
bacterial RNase Ps retains its catalytic activity in the absence of the protein subunit, i.e.
it is a ribozyme. Isolated eukaryotic and archaeal RNase P RNA has not been shown
to retain its catalytic function, but is still essential for the catalytic activity of the
holoenzyme. Although the archaeal and eukaryotic holoenzyme s have a much greater protein content than
the bacterial ones, the RNA cores from all the three lineages are homologous -- helices
corresponding to P1, P2, P3, P4, and P10/11 are common to all cellular RNase P
RNAs. Yet, there is considerable sequence variation, particularly among the eukaryotic RNAs.

Score:

312.6

E-value:

3.5e-102

From sequence:

1

To sequence:

377

Alignments:

                                   v                                    v                                            NC
              {{{{{{{{{{{{{{{{{{,<<<<<<<<<__..................____>>>>>>>>>,,,,,,,,,,,,,[[[[--------[[[[[<<<<<_____> CS
  RF00010   1 cgagccggccgggcggucGCgcccccccuu..................aaaagggggggcGAGGAAAGUCCGGgCUcCAuAGggCAggguGccgGgUAACgc 84 
              ::A C:G:CC:G:C:GUCGC: C::C::                    A   ::G::G :GAGGAAAGUCCGGGCUCCAUAGGGCAGGGUGCC:GGUAACGC
     rnpB   1 GAAGCUGACCAGACAGUCGCCGCUUCGUCGucguccucuucgggggagACGGGCGGAGGGGAGGAAAGUCCGGGCUCCAUAGGGCAGGGUGCCAGGUAACGC 102
              ****************************999999999999999999998888************************************************** PP

                                                                 v             v                                     NC
              >>>><<<<<____>>>>->((---(((((,,,,,,,,,,,,<<<<<..<<<<<<.....____>>>>>>>>>-->>,,,,,,,<<<<<<_______>>>>>> CS
  RF00010  85 CcggcggggGugAccccAgGGAaAGugCcACAGAAAauAgACCgCc..cgcccc.....uuauggggcggGcAAGGGUGAAAaGGugcgGUAAGAGcgcaCC 179
              C:GG:GGG G +A CCCA:G:  AGU:C:ACAGA+A++A+ACC:CC  :G  CC         GG  C:GG:AAGGGUGAAA GGUGCGGUAAGAGCGCACC
     rnpB 103 CUGGGGGG-GAAA-CCCACGACCAGUGCAACAGAGAGCAAACCGCCgaUGGCCCgcgcaAGCGGGAUCAGGUAAGGGUGAAAGGGUGCGGUAAGAGCGCACC 202
              *****665.7777.5668****************************88666666777765555666677********************************* PP

                                                                                                                     NC
              <<<<<<<<<____>>>.>>>>>->,,)))--))))]]]]]]]]],,,<<<<------<<<<<<-----<<<<<_____>>>>>->>>>>>----->>>>,,, CS
  RF00010 180 GcgcccccGGuaACgg.gggcgGCuugGcAAaCCCCacccGgAGCAAggccAAAUAGGggcgcauuuacgggcugcucgcccgagcgccCGGGUaggccGCU 280
              GC C::C:GGUAAC:G ::G GGC+ :G:AAAC:CCACCCGGAGCAA:GCCAAAUAGGGG::CAU++  G::C+GC+CG::C  G::CCCGGGUAGGC:GCU
     rnpB 203 GCGCGGCUGGUAACAGuCCGUGGCACGGUAAACUCCACCCGGAGCAAGGCCAAAUAGGGGUUCAUAA-GGUACGGCCCGUACU-GAACCCGGGUAGGCUGCU 302
              ****************99************************************************8.99999*****99999.****************** PP

                                                                                                      NC
              ,,,<<<<<<<<____>>>>>>>>,,,,,,,,,,}}}}}}}-------------------------------}-}}}}}}}}}}:::: CS
  RF00010 281 uGAGccggccgGuAAcggccggCCuAGAugAAUgaccgccaucuauuuaaauuuuaACAGAAcCCGGCUUAcaggccggcucgucau 367
              UGAGCC:G: :G++A: :C:GGCCUAGAUGAAUGAC:G:C++C            +ACAGAACCCGGCUUA: GG:C:G U::+C+U
     rnpB 303 UGAGCCAGUGAGCGAUUGCUGGCCUAGAUGAAUGACUGUCCAC------------GACAGAACCCGGCUUAUCGGUCAGUUUCACCU 377
              ******************************************9............******************************** PP

RNAfold
>rnpB
AAGCUGACCAGACAGUCGCCGCUUCGUCGUCGUCCUCUUCGGGGGAGACG
GGCGGAGGGGAGGAAAGUCCGGGCUCCAUAGGGCAGGGUGCCAGGUAACG
CCUGGGGGGGAAACCCACGACCAGUGCAACAGAGAGCAAACCGCCGAUGG
CCCGCGCAAGCGGGAUCAGGUAAGGGUGAAAGGGUGCGGUAAGAGCGCAC
CGCGCGGCUGGUAACAGUCCGUGGCACGGUAAACUCCACCCGGAGCAAGG
CCAAAUAGGGGUUCAUAAGGUACGGCCCGUACUGAACCCGGGUAGGCUGC
UUGAGCCAGUGAGCGAUUGCUGGCCUAGAUGAAUGACUGUCCACGACAGA
ACCCGGCUUAUCGGUCAGUUUCACCU
(((((((((..((..((.((.((((((((((.(((((....)))))))).))))))).))..))..))((..(((((...(((..((((.((((((...)))))).(((....)))...(((.((((..............((((..((((((((....))))).)))......))))....((((((.......)))))).((((((((....))).))))))))))))..))))..))))))))..))........(((((((....(((((...)))))))))))).(((((((((((.(.((((((((....))))))))).))........(((((...)))))....))))))))))))))))))..... (-160.40)

Length sequence

: 376

Length match:

375